Project objectiveBiCT was responsible for the mutagenesis, production and purification of the enzyme at the heart of the project.
It optimised the enzymatic step for the stereoselective production of the intermediate of interest from a racemic mixture, with particular attention to reaction time, enzyme/substrate ratio and transformation yield. He has also developed a strategy for recovering the acid of interest from the reaction mixture for the racemization step.
Finally, he took part in the basic engineering of the process demonstration plant, defining and installing a demonstration subunit for the production of the biocatalyst.
Project descriptionThe INCITE project will contribute to the introduction of biotechnology in the production of agrochemicals by designing demonstration plants for 2 chemo-enzymatic processes.
The first process involves the production of an intermediate using an enzyme, which can promote the reuse of chemicals involved in the process.
The second case concerns the solvent-free synthesis of esters using enzymes.
BiCT is involved in process development and enzyme production.